Volume 14 - 2023 Issue 1
1. Endophytic fungi in green manure crops; friends or foe?
Abeywickrama PD et al. (2023)
Volume 13 - 2022 Issue 2 (SI Fungal Evolution)
9. Special Issue: Fungal Evolution, in honour of the Academician Professor Yu Li’s 80th Birthday
Hyde Kevin David et al. (2023)
5. Comparative genomics provides new insights into the evolution of Colletotrichum
Chen YP et al. (2022)
4. Large-scale genome investigations reveal insights into domestication of cultivated mushrooms
Fu YP et al. (2022)
2. Phylogenetic diversity and affiliation of tropical African ectomycorrhizal fungi
Houdanon RD et al. (2022)
Volume 8 - 2017 - Issue 5 (SI Diaporthe)-closed
1. Diaporthe species on Rosaceae with descriptions of D. pyracanthae sp. nov. and D. malorum sp. nov.
Authors: Santos L, Phillips AJL, Crous P, Alves A
Recieved: 24 January 2017, Accepted: 03 March 2017, Published: 12 March 2017
The family Rosaceae includes a large number of species ranging from herbaceous (Fragaria) to ornamental plants (Rosa and Pyracantha) and fruit trees (Malus and Pyrus). Diaporthe species have been associated with twig canker, shoot blight, dieback, wood decay and fruit rot on members of the Rosaceae. In this study a collection of isolates from several Rosaceae hosts were characterised by multi-locus sequence analyses using the internal transcribed spacer, translation elongation factor 1-alpha, beta-tubulin, histone H3 and calmodulin loci. The phylogenetic analyses of the combined five loci revealed that the isolates studied were distributed among four clades, of which two correspond to D. foeniculina and D. eres. The other two clades, closely related to D. passiflorae and D. leucospermi represent two new species, D. pyracanthae sp. nov. and D. malorum sp. nov., respectively. Further, pathogenicity assays have shown that of the four species tested, D. malorum was the most aggressive species on apple fruit and D. eres was the most aggressive species on detached pear twigs. A revision of all Diaporthe (and Phomopsis) names that have been associated with Rosaceae hosts as well as their current status as pathogens of members of this family is presented.
Keywords: Malus – Pathogenicity – Phylogeny – Pyracantha – Pyrus
Authors: Dissanayake AJ, Zhang W, Liu M, Hyde KD, Zhao W, Li XH, Yan JY
Recieved: 26 February 2017, Accepted: 05 March 2017, Published: 03 April 2017
Peach tree diseases have a variety of symptoms and causes. Only Botryosphaeriaceae taxa have been reported in association with peach trees in Chinese peach orchards. This study aims to identify and characterize Diaporthe species associated with peach trees in Jinshui Experimental Orchard in Hubei Academy of Agriculture Sciences, Hubei Province, China. The fungi were isolated from diseased peach trunks and shoots showing exudates. Fungal identification was accomplished using a combination of morphological and pathogenic characteristics together with phylogenetic analyses based on internal transcribed spacer (ITS), partial translation elongation factor 1-α (EF1-α), β-tubulin (BT) and calmodulin (CAL) sequences. A total of 48 Diaporthe isolates were obtained from 62 diseased samples and most isolates were identified as Diaporthe eres (69 %), followed by D. momicola sp. nov (12.5 %), D. pescicola sp. nov. (10 %) and D. taoicola sp. nov. (8.5 %). All identified species were able to cause necrotic lesions at different levels of severity when inoculated into detached peach shoots.
Keywords: Diaporthaceae – Morphology – Multi-gene phylogeny – Pathogenicity – Prunus persica – Taxonomy
3. Diaporthe juglandicola sp. nov. (Diaporthales, Ascomycetes), evidenced by morphological characters and phylogenetic analysis
Authors: Yang Q, Fan XL, Du Z, Tian CM
Recieved: 06 April 2017, Accepted: 18 May 2017, Published: 08 June 2017
Diaporthe juglandicola sp. nov, collected from diseased branches of Juglans mandshurica in Beijing, China, is described and illustrated in this paper. Evidence for this new species is provided by its holomorphic morphology and phylogenetic analysis. Morphologically, the asexual morph produces hyaline, aseptate, ellipsoidal, alpha conidia (8.1–8.7 × 2.3–2.9 μm), while the sexual morph produces 8-spored, unitunicate, clavate to cylindric asci and fusoid, 0–1-septate ascospores. The phylogeny inferred from combined multi-locus sequences (CAL, HIS, ITS, TEF1-α, TUB) grouped the isolates of the new species into a distinct lineage.
Keywords: dieback – molecular phylogeny – new species – taxonomy
Authors: Dissanayake AJ, Camporesi E, Hyde KD, Zhang Wei, Yan JY, Li XH
Recieved: 21 May 2017, Accepted: 06 June 2017, Published: 12 June 2017
Seven new species of Diaporthe, D. acericola on Acer negundo, D. cichorii on Cichorium intybus, D. dorycnii on Dorycnium hirsutum, D. lonicerae on Lonicera sp., Laurus nobilis and Torilis arvensis, D. pseudotsugae on Pseudotsuga menziesii, D. schoeni on Schoenus nigricans, Carduus sp. and Plantago sp. and D. torilicola on Torilis arvensis from Italy are described and illustrated based on morphological characteristics and molecular analyses. In addition to the new species, eight known species of Diaporthe, D. eres, D. foeniculina, D. gulyae, D. novem, D. ravennica, D. rhusicola, D. rudis and D. sterilis were identified. Phylogenetic relationships of the new species with other Diaporthe species were revealed by DNA sequence analyses based on the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF), partial regions of the β-tubulin (BT) and calmodulin (CAL). Among 44 isolates, D. eres was the dominant species, accounting for 27% of the frequency of occurrence. Our study revealed a high diversity of undescribed Diaporthe species from various hosts in Italy.
Keywords: Diaporthales – Hosts – Morphology – Sordariomycetes – Taxonomy
Authors: Dissanayake AJ, Phillips AJL, Hyde KD, Yan JY, Li XH
Recieved: 28 June 2017, Accepted: 17 July 2017, Published: 06 August 2017
In this paper we give an account of species in the genus Diaporthe. Since morphological characters are inadequate to define species in this genus, DNA sequence data are essential to differentiate them. We therefore focus this paper on the 171 species for which ex-type/exepitype/ex-isotype/ex-neotype isolates and corresponding molecular data are available and these species are listed alphabetically. Sexual or asexual morph are noted under each species, detailed descriptions of type materials, host records and geographic distribution are provided. Available DNA sequence data from ex-type cultures are listed in Table 1. Phylogenetic relationships of the species are given in a multi-locus phylogenetic tree based on combined ITS, tef1-α, β-tubulin and CAL sequences.
Keywords: asexual morph– distribution – hosts – sexual morph – type species
6. Diaporthe toxicodendri sp. nov., a causal fungus of the canker disease on Toxicodendron vernicifluum in Japan
Authors: Ando Y, Masuya H, Aikawa T, Ichihara Y, Tabata M
Recieved: 05 November 2017, Accepted: 02 November 2017, Published: 28 November 2017
We describe for the first time the fungus Diaporthe toxicodendri sp. nov., which causes canker disease on the stems and twigs of Toxicodendron vernicifluum. We conducted a phylogenetic analysis using combined multigene sequence data from the rDNA internal transcribed spacer sequence and partial genes for calmodulin, histone H3, beta-tubulin, and translation elongation factor 1-alpha. The results indicate that D. toxicodendri occupies a monophyletic clade with high support. Although 10 species are phylogenetically closely related to D. toxicodendri, morphological characteristics of size of alpha conidia and lacking of beta and gamma conidia support the distinction of this fungus from those closely related species. No sexual morphic structures have yet been found for the species. The pathogenicity of this species was confirmed by the inoculation test to T. vernicifluum.
Keywords: Anacardiaceae – canker disease – Diaporthales – Phomopsis – taxonomy