Volume 8 - 2017 - Issue 2 (SI Botryosphaeriales)-closed

1. Special issue – Towards a better understanding of Botryosphaeriales

Authors: AJL Phillips

Recieved: 07 February 2017, Accepted: 08 February 2017, Published: 08 February 2017



2. Botryosphaeria rosaceae sp. nov. and B. ramosa, new botryosphaeriaceous taxa from China

Authors: Zhou YP, Zhang M, Dou ZhP, Zhang Y

Recieved: 21 December 2016, Accepted: 29 December 2016, Published: 17 February 2017

A new species of Botryosphaeria (B. rosaceae) is described and illustrated from Rosaceae in China. Phylogenetic analysis based on combined ITS, LSU, tef1-α and TUB loci supported its separation from another species of Botryosphaeria. Morphologically, B. rosaceae was comparable with B. sinensia, but the smaller and slenderer ascospores and conidia as well as the broader host spectrum of B. sinensia differentiate it from B. rosaceae. In addition, Botryosphaeria ramosa was found associated with an Acacia species in this study, which is a new host and first report in the Northern Hemisphere. 

Keywords: Asia – Multilocus phylogeny – Rosaceae – Taxonomy


3. Lasiodiplodia chinensis, a new holomorphic species from China

Authors: Dou ZP, He W, Zhang Y

Recieved: 01 January 2017, Accepted: 16 March 2017, Published: 27 March 2017

A new species of Lasiodiplodia (L. chinensis) is described and illustrated from several hosts collected from Hainan and Shandong Province in China. Both sexual and asexual states of L. chinensis were observed, which is characterized by its broadly clavate to clavate asci, fusiform, hyaline and aseptate ascospores, and initially hyaline, aseptate, ovoid to ellipsoid conidia that become pigmented and 1–2-septate with longitudinal striations when mature. Phylogenetically, L. chinensis is closely related to L. pseudotheobromae, L. sterculiae and L. lignicola. Morphological comparisons of these four species lead to the conclusion that the collected taxon is new to science

Keywords: Botryosphaeriaceae – phylogeny – sexual morph – taxonomy


4. Three species of Neofusicoccum (Botryosphaeriaceae, Botryosphaeriales) associated with woody plants from southern China

Authors: Zhang M, Lin S, He W, Zhang Y

Recieved: 25 February 2017, Accepted: 08 March 2017, Published: 25 May 2017

Two new species, namely N. sinense and N. illicii, collected from Guizhou and Guangxi provinces in China, are described and illustrated. Phylogenetic analysis based on combined ITS, tef1-α and TUB loci supported their separation from other reported species of Neofusicoccum. Morphologically, the relatively large conidia of N. illicii, which become 1–3-septate and pale yellow when aged, can be distinguishable from all other reported species of Neofusicoccum. Phylogenetically, N. sinense is closely related to N. brasiliense, N. grevilleae and N. kwambonambiense. The smaller conidia of N. sinense, which have lower L/W ratio and become 1–2-septate when aged, differ from the other three species. Neofusicoccum mangiferae was isolated from the dieback symptoms of mango in Guangdong Province.

Keywords: Asia – endophytes – Morphology– Taxonomy


5. Does morphology matter in taxonomy of Lasiodiplodia? An answer from Lasiodiplodia hyalina sp. nov.

Authors: Dou ZP, He W, Zhang Y

Recieved: 15 April 2017, Accepted: 01 June 2017, Published: 07 July 2017

A new species of Lasiodiplodia (L. hyalina) is described and illustrated from Acacia confusa and an unidentified woody plant collected in Southern China. Only asexual states of L. hyalina were observed, which is characterized by most conidia remaining hyaline with only about 10% conidia becoming pigmented after three months in culture. Phylogenetically, L. hyalina is closely related to L. thailandica. Morphologically, the larger conidiogenous cells and paraphyses of L. hyalina are distinct from those of L. thailandica, which leads to the conclusion that the collected taxon is new to science. Lasiodiplodia thailandica is reported as a new record in China with Podocarpus macrophyllus and Albizia chinensis as its new hosts.

Keywords: Botryosphaeriaceae – China – hyaline conidia – phylogeny


6. Dothiorella magnoliae, a new species associated with dieback of Magnolia grandiflora from China

Authors: You CJ, Liu X, Li LX, Tsui CKM, Tian CM

Recieved: 04 May 2017, Accepted: 25 May 2017, Published: 24 July 2017

Dothiorella magnoliae sp. nov. was identified and isolated from Magnolia grandiflora from Sichuan Province in China. The new taxon is described and illustrated based on unique morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS) rDNA, and partial sequences of the elongation factor 1–α (tef1-α) gene. Morphologically, D. magnoliae produces conidia with conspicuous constriction at septum and it also differs from other described Dothiorella species in the dimensions of conidia. Molecular data reveal that D. magnoliae forms a sister clade to other species of Dothiorella, thus a new species is introduced here. 



7. Saprobic Botryosphaeriaceae, including Dothiorella italica sp. nov., associated with urban and forest trees in Italy

Authors: Dissanayake AJ, Camporesi E, Hyde KD, Yan JY, Li XH

Recieved: 28 June 2017, Accepted: 17 July 2017, Published: 06 August 2017

A collection of saprobic botryosphaeriaceous isolates (from dead aerial branches, stems, and dead leaves) was obtain from urban and forest tree species in Italy. A total of 52 isolates were characterized by multi-locus sequence analysis of the internal transcribed spacer region (ITS) and partial sequences of the translation elongation factor 1-alpha (TEF). According to morphological characteristics and analysis of multi-gene sequence data, 14 species in Botryosphaeriaceae belonging to five genera, viz. Botryosphaeria, Diplodia, Dothiorella, Eutiarosporella and Neofusicoccum were identified, including Dothiorella italica sp. nov. The other known species isolated were Botryosphaeria dothidea, B. auasmontanum, Diplodia alatafructa, Di. crataegicola, Di. mutila, Di. sapinea, Di. seriata, Dothiorella omnivora, Do. rhamni, Do. sarmentorum, Do. sempervirentis, Eutiarosporella dactylidis and Neofusicoccum parvum. Of these, Botryosphaeria dothidea and Diplodia seriata were the most prevalent species among the isolates. Several new host-fungus relationships were established. This study indicates the fungal family Botryosphaeriaceae seems to be common and widespread on a broad range of hosts in Italy.

Keywords: Botryosphaeriales – Botryosphaeria – Diplodia – Eutiarosporella – multigene phylogeny – Neofusicoccum


8. Aplosporella ginkgonis (Aplosporellaceae, Botryosphaeriales), a new species isolated from Ginkgo biloba in China

Authors: Du Z, Fan XL, Yang Q, Hyde KD, Tian CM

Recieved: 30 April 2017, Accepted: 28 July 2017, Published: 18 August 2017

Aplosporella ginkgonis sp. nov., is described from symptomatic branches of Ginkgo biloba in China based on morphological and molecular analysis. It is characterized by multiloculate conidiomata, with one to four ostioles, and aseptate, brown, ellipsoid to oblong conidia. Morphological and phylogenetic analyses of ITS, and tef1-α sequence data, support the position of the new species in Aplosporella, which forms a monophyletic lineage with strong support (MP/BI = 100/1). Thus, a new species is introduced in this paper to accommodate this taxon.

Keywords: Ascomycetous fungi – Canker disease – Dothideomycetes – Systematics – Taxonomy


9. Reaction of some apple cultivars to Diplodia bulgarica in Iran

Authors: Hanifeh S, Zafari D and Soleimani MJ

Recieved: 13 March 2017, Accepted: 09 October 2017, Published: 17 October 2017

In recent years, Diplodia bulgarica A.J.L. Phillips, J. Lopes & S.G. Bobev was identified as the main causal agent of apple tree decline in Iran. This fungus causes stem and trunk cankers, dieback, bark discoloration, gummosis, scaling-off of the bark and fruit rot in the west and northwest apple orchards of Iran. The disease was often prevalent in trees more than 15-years-old that were suffering from environmental stresses such as drought and nutrient deficiency. To evaluate the response of apple tree cultivars to this pathogen, six commercial cultivars (Golden Delicious, Red Delicious, Granny Smith, Red Starking, Braeburn, and Gala) and three local cultivars (Golab, Qara Yapragh, and Turkaman) were assessed under field conditions. The results confirmed that all cultivars are susceptible to Diplodia bulgarica, but mean lesion lengths differed significantly between cultivars (P=0.01). The largest lesions were observed on Braeburn, Red Delicious, and Red Starking cultivars, thus these cultivars were considered to be the most susceptible. Lesion lengths on Qara Yapragh, Turkaman, and Golden Delicious were significantly smaller than on all other cultivars and these were considered as moderately resistant to Diplodia bulgarica in Iran. To our knowledge, this is the first report on the response of apple tree cultivars to this devastating pathogen.

Keywords: diplodia canker – gummosis – susceptible


10. Molecular characterization and pathogenicity of Diplodia corticola and other Botryosphaeriaceae species associated with canker and dieback of Quercus suber in Algeria

Authors: Smahi H, Belhoucine-Guezouli L, Berraf-Tebbal A, Chouih S, Arkam M, Franceschini A, Linaldeddu BT, Phillips AJL

Recieved: 26 July 2017, Accepted: 02 November 2017, Published: 03 November 2017

In Algeria cork oak forests are irregularly distributed in the northern regions along the Tell Atlas mountain range. Over the last few decades, severe tree decline and mortality events has been observed in several of the main cork oak forests. Since there is little information about the aetiology of this decline and given the high ecological and economic importance of cork oak ecosystems, a survey was carried out in six forests, M’sila and Hafir (northwestern Algeria), and Ksar Fatma, Haddada, Aïn Zana and Oued El Hout (northeastern Algeria), to establish the fungal pathogens associated with cork oak branch diseases. Isolations from symptomatic branches of 88 declining trees yielded a total of 96 fungal isolates of which 69 belonged to three distinct genera of Botryosphaeriaceae, namely Diplodia, Dothiorella and Lasiodiplodia. On the basis of morphological features and DNA sequence data (ITS and tef1-α), five species: Diplodia corticola, D. quercivora, D. sapinea, Dothiorella iberica and Lasiodiplodia exigua were identified. The geographical distribution and occurrence of the five species differed greatly among sites. The occurrence of both known lineages of D. corticola was also recognised. Pathogenicity trials showed that all the species assayed are pathogenic on cork oak and D. corticola proved to be the most aggressive.

Keywords: complex aetiology – Diplodia corticola – evolutionary lineages – oak decline


11. Two new endophytic species of Phyllosticta (Phyllostictaceae, Botryosphaeriales) from Southern China

Authors: Lin S, Sun X, He W, Zhang Y

Recieved: 13 September 2017, Accepted: 02 November 2017, Published: 06 November 2017

Phyllosticta is an important genus known to cause various leaf spots and fruit diseases worldwide on a large range of hosts. Two new endophytic species of Phyllosticta (P. dendrobii and P. illicii) are described and illustrated from Dendrobium nobile and Illicium verum in China. Phylogenetic analysis based on combined ITS, LSU, tef1-a, ACT and GPDH loci supported their separation from other species of Phyllosticta. Morphologically, P. dendrobii is most comparable with P. aplectri, while the large-sized pycnidia of P. dendrobii differentiate it from P. aplectri. Members of Phyllosticta are first reported from Dendrobium and Illicium. 

Keywords: Asia – Botryosphaeriales – leaf spots – Multilocus phylogeny


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Mycosphere publishes reviews, research articles, methodology papers, taxonomic works such as monographs, which are relevant to fungal biology, including lichens. The official journal language is English.

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