Volume 8 - Issue 5 (SI Diaporthe)-closed

Article Number 1

Diaporthe species on Rosaceae with descriptions of D. pyracanthae sp. nov. and D. malorum sp. nov.


Santos L, Phillips AJL, Crous P, Alves A

Received 24 January 2017
Accepted 03 March 2017
Published Online 12 March 2017
Corresponding Author Artur Alves – artur.alves@ua.pt

The family Rosaceae includes a large number of species ranging from herbaceous (Fragaria) to ornamental plants (Rosa and Pyracantha) and fruit trees (Malus and Pyrus). Diaporthe species have been associated with twig canker, shoot blight, dieback, wood decay and fruit rot on members of the Rosaceae. In this study a collection of isolates from several Rosaceae hosts were characterised by multi-locus sequence analyses using the internal transcribed spacer, translation elongation factor 1-alpha, beta-tubulin, histone H3 and calmodulin loci. The phylogenetic analyses of the combined five loci revealed that the isolates studied were distributed among four clades, of which two correspond to D. foeniculina and D. eres. The other two clades, closely related to D. passiflorae and D. leucospermi represent two new species, D. pyracanthae sp. nov. and D. malorum sp. nov., respectively. Further, pathogenicity assays have shown that of the four species tested, D. malorum was the most aggressive species on apple fruit and D. eres was the most aggressive species on detached pear twigs. A revision of all Diaporthe (and Phomopsis) names that have been associated with Rosaceae hosts as well as their current status as pathogens of members of this family is presented.

Keywords Malus – Pathogenicity – Phylogeny – Pyracantha – Pyrus
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Article Number 2

Diaporthe species associated with peach tree dieback in Hubei, China


Dissanayake AJ, Zhang W, Liu M, Hyde KD, Zhao W, Li XH, Yan JY

Received 26 February 2017
Accepted 05 March 2017
Published Online 03 April 2017
Corresponding Author J.Y. Yan – jiyeyan@vip.163.com, X.H. Li – lixinghong1962@163.com

Peach tree diseases have a variety of symptoms and causes. Only Botryosphaeriaceae taxa have been reported in association with peach trees in Chinese peach orchards. This study aims to identify and characterize Diaporthe species associated with peach trees in Jinshui Experimental Orchard in Hubei Academy of Agriculture Sciences, Hubei Province, China. The fungi were isolated from diseased peach trunks and shoots showing exudates. Fungal identification was accomplished using a combination of morphological and pathogenic characteristics together with phylogenetic analyses based on internal transcribed spacer (ITS), partial translation elongation factor 1-α (EF1-α), β-tubulin (BT) and calmodulin (CAL) sequences. A total of 48 Diaporthe isolates were obtained from 62 diseased samples and most isolates were identified as Diaporthe eres (69 %), followed by D. momicola sp. nov (12.5 %), D. pescicola sp. nov. (10 %) and D. taoicola sp. nov. (8.5 %). All identified species were able to cause necrotic lesions at different levels of severity when inoculated into detached peach shoots.

Keywords Diaporthaceae – Morphology – Multi-gene phylogeny – Pathogenicity – Prunus persica – Taxonomy
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Article Number 3

Diaporthe juglandicola sp. nov. (Diaporthales, Ascomycetes), evidenced by morphological characters and phylogenetic analysis


Yang Q, Fan XL, Du Z, Tian CM

Received 06 April 2017
Accepted 18 May 2017
Published Online 08 June 2017
Corresponding Author Cheng M. Tian – chengmt@bjfu.edu.cn

Diaporthe juglandicola sp. nov, collected from diseased branches of Juglans mandshurica in Beijing, China, is described and illustrated in this paper. Evidence for this new species is provided by its holomorphic morphology and phylogenetic analysis. Morphologically, the asexual morph produces hyaline, aseptate, ellipsoidal, alpha conidia (8.1–8.7 × 2.3–2.9 μm), while the sexual morph produces 8-spored, unitunicate, clavate to cylindric asci and fusoid, 0–1-septate ascospores. The phylogeny inferred from combined multi-locus sequences (CAL, HIS, ITS, TEF1-α, TUB) grouped the isolates of the new species into a distinct lineage.

Keywords dieback – molecular phylogeny – new species – taxonomy
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Article Number 4

Molecular phylogenetic analysis reveals seven new Diaporthe species from Italy


Dissanayake AJ, Camporesi E, Hyde KD, Zhang Wei, Yan JY, Li XH

Received 21 May 2017
Accepted 06 June 2017
Published Online 12 June 2017
Corresponding Author Xinghong Li - lixinghong1962@163.com

Seven new species of Diaporthe, D. acericola on Acer negundo, D. cichorii on Cichorium intybus, D. dorycnii on Dorycnium hirsutum, D. lonicerae on Lonicera sp., Laurus nobilis and Torilis arvensis, D. pseudotsugae on Pseudotsuga menziesii, D. schoeni on Schoenus nigricans, Carduus sp. and Plantago sp. and D. torilicola on Torilis arvensis from Italy are described and illustrated based on morphological characteristics and molecular analyses. In addition to the new species, eight known species of Diaporthe, D. eres, D. foeniculina, D. gulyae, D. novem, D. ravennica, D. rhusicola, D. rudis and D. sterilis were identified. Phylogenetic relationships of the new species with other Diaporthe species were revealed by DNA sequence analyses based on the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF), partial regions of the β-tubulin (BT) and calmodulin (CAL). Among 44 isolates, D. eres was the dominant species, accounting for 27% of the frequency of occurrence. Our study revealed a high diversity of undescribed Diaporthe species from various hosts in Italy.

Keywords Diaporthales – Hosts – Morphology – Sordariomycetes – Taxonomy
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Article Number 5

The current status of species in Diaporthe


Dissanayake AJ, Phillips AJL, Hyde KD, Yan JY, Li XH

Received 28 June 2017
Accepted 17 July 2017
Published Online 06 August 2017
Corresponding Author XingHong Li – e-mail – lixinghong1962@163.com

In this paper we give an account of species in the genus Diaporthe. Since morphological characters are inadequate to define species in this genus, DNA sequence data are essential to differentiate them. We therefore focus this paper on the 171 species for which ex-type/exepitype/ex-isotype/ex-neotype isolates and corresponding molecular data are available and these species are listed alphabetically. Sexual or asexual morph are noted under each species, detailed descriptions of type materials, host records and geographic distribution are provided. Available DNA sequence data from ex-type cultures are listed in Table 1. Phylogenetic relationships of the species are given in a multi-locus phylogenetic tree based on combined ITS, tef1-α, β-tubulin and CAL sequences.  

Keywords asexual morph– distribution – hosts – sexual morph – type species
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Article Number 6

Diaporthe toxicodendri sp. nov., a causal fungus of the canker disease on Toxicodendron vernicifluum in Japan


Ando Y, Masuya H, Aikawa T, Ichihara Y, Tabata M

Received 05 November 2017
Accepted 02 November 2017
Published Online 28 November 2017
Corresponding Author Masanobu Tabata – e-mail – butter@ffpri.affrc.go.jp

We describe for the first time the fungus Diaporthe toxicodendri sp. nov., which causes canker disease on the stems and twigs of Toxicodendron vernicifluum. We conducted a phylogenetic analysis using combined multigene sequence data from the rDNA internal transcribed spacer sequence and partial genes for calmodulin, histone H3, beta-tubulin, and translation elongation factor 1-alpha. The results indicate that D. toxicodendri occupies a monophyletic clade with high support. Although 10 species are phylogenetically closely related to D. toxicodendri, morphological characteristics of size of alpha conidia and lacking of beta and gamma conidia support the distinction of this fungus from those closely related species. No sexual morphic structures have yet been found for the species. The pathogenicity of this species was confirmed by the inoculation test to T. vernicifluum.

Keywords Anacardiaceae – canker disease – Diaporthales – Phomopsis – taxonomy
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