Volume 8 - Issue 7 (May/June)


Article Number 1
Title

A new species of Conidiobolus with chlamydospores from Dabie Mountains, Eastern China

Authors

Nie Y, Tang XX, Liu XY, Huang B

Received 05 March 2017
Accepted 25 May 2017
Published Online 29 May 2017
Corresponding Author Huang B – bhuang@ahau.edu.cn, Liu XY – e-mail – Liuxiaoyong@im.ac.cn
Abstract

Conidiobolus is a widely distributed genus throughout the world, and its resting spores include zygospores, chlamydospores and villose conidia. Conidiobolus dabieshanensis is confirmed as a new species with chlamydospores within the genus Conidiobolus based on the morphological evidence and the molecular data of LSU rDNA region. It is morphologically allied with C. adiaeretus and C. firmipilleus and it is phylogenetically closely related to C. humicolus, C. chlamydosporus and C. firmipilleus. The new species differs from C. adiaeretus by the absence of capilliconidia, from C. firmipilleus and C. chlamydosporus by its size of primary conidia and slender and curving germ hyphae arising from a single conidium, and from C. humicolus by its larger primary conidia and the absence of zygospores.

Keywords Entomophthorales – Morphology – Molecular phylogeny
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Article Number 2
Title

Sarocladium brachiariae sp. nov., an endophytic fungus isolated from Brachiaria brizantha

Authors

Liu XB, Guo ZK, Huang GX

Received 29 March 2017
Accepted 15 May 2017
Published Online 09 June 2017
Corresponding Author Gui-Xiu Huang – hgxiu@vip.163.com
Abstract

In a survey on the diversity of endophytic fungi of Brachiaria brizantha, a new species of Sarocladium was isolated and proposed here as Sarocladium brachiariae. According to the LSU and ITS rDNA sequences and culture morphology and micromorphology, the species differed from the species hitherto described in Sarocladium, and is characterized as a new species. The new species can produce hyphal coils and slimy heads. In addition, raised, cottony, moist to slimy colonies on PDA, and adelophialides, branched conidiophores were also useful characters for distinguishing species from other species of Sarocladium.

Keywords Endophytes – Sarocladium – Phylogeny – Taxonomy
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Article Number 3
Title

Further characterization and pathogenicity of Didymella microchlamydospora causing stem necrosis of Morus nigra in Iran

Authors

Ahmadpour SA, Farokhinejad R, Mehrabi-Koushki M

Received 10 March 2017
Accepted 11 April 2017
Published Online 09 June 2017
Corresponding Author Mehdi Mehrabi-Koushki – mhdmhrb@scu.ac.ir; mhdmhrb@gmail.com
Abstract

In the last decade, canker and dieback diseases have caused disease of ornamental and fruit trees of Khuzestan Province in the southwest of Iran. Fourty-eight symptomatic branches and trunks were sampled and a survey was made to identify the probable pathogens, which led to the isolation of the recently established species, Didymella microchlamydospora. A multi-locus DNA sequence based phylogeny, in combination with morphology, was used to characterize seven isolates of this species. Two phylogenetic trees constructed based on the combined sequences of ITS/LSU/tub2 and ITS/LSU/tub2/rpb2 regions showed very little differences, and both trees presented generally consistent relationships among the strongly supported clades. In both of three- and four-locus based phylogenetic trees, our isolates and a reference strain, D. microchlamydospora CBS 105.95, formed supportive monophyletic clades with strong 99% and 100% BS support, respectively. In pathogenicity tests, the isolate of D. microchlamydospora SCUA 14_Dez_Mor formed the necrosis and wood discoloration on stem fragments of Morus nigra. To our knowledge, this is the first report of pathogenicity of D. microchlamydospora on Morus nigra and its association on plants of olive, bitter orange, oleander and bottlebrush worldwide. In addition, we gave a slightly amended description of this species.

Keywords die back – Khuzestan – multi-locus phylogeny
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Article Number 4
Title

A new species of Antrodia (Basidiomycota, Polypores) from China

Authors

Chen YY, Wu F

Received 22 February 2017
Accepted 24 May 2017
Published Online 12 June 2017
Corresponding Author Fang Wu –fangwubjfu2014@yahoo.com
Abstract

A new species, Antrodia monomitica sp. nov., is described and illustrated from China based on morphological characters and molecular evidence. It is characterized by producing annual, fragile and nodulose basidiomata, a monomitic hyphal system with clamp connections on generative hyphae, hyaline, thin-walled and fusiform to mango-shaped basidiospores (6–7.5 × 2.3–3 μm), and causing a typical brown rot. In phylogenetic analysis inferred from ITS and nLSU rDNA sequences, the new species forms a distinct lineage in the Antrodia s. l., and has a close relationship with A. oleracea.

Keywords Fomitopsidaceae – phylogenetic analysis – taxonomy – wood-decaying fungi
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Article Number 5
Title

Effects of additives and bioreactors on cordycepin production from Cordyceps militaris in liquid static culture

Authors

Wen TC, Long FY, Kang C, Wang F, Zeng W

Received 11 April 2017
Accepted 15 May 2017
Published Online 12 June 2017
Corresponding Author Chao Kang – e-mail –camlink@qq.com
Abstract

Cordycepin (3′-deoxyadenosine), a nucleoside analog, was isolated from Cordyceps militaris, an entomopathogenic fungus, important in Traditional Chinese Medicine. In this study, Cordycepin production by three strains of C. militaris (strains GACP08Y5, GACP08Y1 and GACP0746) in static liquid culture was established using different working volumes and bioreactors. The best cordycepin production of 3005.83 mg/L was obtained by strain GACP08Y5 in 5 L-flasks, containing 2 L medium at day 40, and total cordycepin content reached 6011.66 mg/flask. The utilization ratio of adenine reached 91%. This is the highest report of cordycepin production in a single fermenter. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

Keywords comparison – cordycepin – Cordyceps militaris – Growth curve – large scale production
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Article Number 6
Title

Genetic variability in Setchelliogaster tenuipes (Setch.) Pouzar based on DNA barcoding ITS

Authors

Sulzbacher MA, Bevilacqua CB, Baldoni DB, Jacques RJS, Antoniolli ZI et al.

Received 13 January 2017
Accepted 15 March 2017
Published Online 12 June 2017
Corresponding Author Marcelo A. Sulzbacher – e-mail – marcelo_sulzbacher@yahoo.com.br
Abstract

Setchelliogaster tenuipes is ectomycorrhizal species with Eucalyptus spp. and currently occurring throughout the world, introduced from Australasia. In South America, it is known from Argentina, Brazil, Uruguay and Chile. Some of the main features of S. tenuipes are the reddish brown basidiomes, presence of stipe, shape of cystidia and peridium structures. The aim of the study is to report the genetic diversity of Setchelliogaster tenuipes (Setch.) Pouzar in Brazil, as well as, in the world distribution. It was presented morphological and phylogenetic notes of this species sampled in Brazil.

Keywords ectomycorrhizal fungi – Eucalyptus plantation – nrDNA – sequestrate fungi
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Article Number 7
Title

A new species and a new combination of Aleurodiscus s.l. (Russulales, Basidiomycota)

Authors

Dai LD, He SH.

Received 09 June 2017
Accepted 10 June 2017
Published Online 16 June 2017
Corresponding Author Shuang-Hui He – shuanghuihe@yahoo.com
Abstract

Aleurodiscus ryvardenii sp. nov. from southwestern China is described and illustrated. The species is characterized by possessing effused basidiocarps, simple-septate generative hyphae, moniliform gloeocystidia and verrucose basidiospores. Aleurodiscus tsugae on coniferous bark in East Asia is transferred to Aleurocystidiellum. Illustrated description of Aleurocystidiellum tsugae and a key to the three species of Aleurocystidiellum are given. The phylogeny of 29 species of Aleurdiscus s.l., Stereum and Xylobolus was inferred from ITS sequences.

Keywords Aleurocystidiellum – ITS – Stereaceae – taxonomy – wood-inhabiting fungi
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Article Number 8
Title

Phylogenetic investigations on freshwater fungi in Tubeufiaceae (Tubeufiales) reveals the new genus Dictyospora and new species Chlamydotubeufia aquatica and Helicosporium flavum

Authors

Brahamanage RS, Lu YZ, Bhat DJ, Wanasinghe DN, Yan JY, Hyde KD, Boonmee S

Received 11 May 2017
Accepted 16 June 2017
Published Online 25 June 2017
Corresponding Author Saranyaphat Boonmee – saranyaphat.khag2@gmail.com
Abstract

Seven new collections of sexual and asexual morphs of Tubeufiaceae from woody substrates in freshwater, were obtained from different regions of Thailand. ITS, LSU and TEF1α sequence data obtained from single spore isolates of these collections were analyzed with other species of Tubeufiaceae. The phylogenetic analyses with combined ITS, LSU and TEF1α data indicated that the collections represented three new species, Chlamydotubeufia aquatica, Dictyospora thailandica and Helicosporium flavum. A new genus, Dictyospora is introduced to accommodate Dictyospora thailandica. Morphological descriptions and illustrations are provided for the new taxa and compared with similar taxa. A single ascospore isolates of Dictyospora thailandica and Helicosporium flavum produced asexual conidia in MEA.

Keywords Chlamydotubeufia – Dictyospora – Helicosporium – lignicolous fungi – phylogeny – taxonomy
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Article Number 9
Title

Mycosphere Essays 19: Recent advances and future challenges in taxonomy of coelomycetous fungi

Authors

Wijayawardene NN, Papizadeh M, Phillips AJL, Wanasinghe DN, Bhat DJ, Weerahewa HLD, Shenoy BD, Wang Y, Huang YQ

Received 09 May 2017
Accepted 09 June 2017
Published Online 26 June 2017
Corresponding Author Yu-Qiang Huang – hyqlch@163.com
Abstract

The application of molecular phylogenetic methods has provided a better understanding of the taxonomy and evolution of coelomycetous fungi. Providing taxonomic placements for orphan genera, re-visiting historic genera, resolving species complexes and polyphyletic genera are progressing with new data continually immerging. Taxonomists need to implement the usage of adopted names of pleomorphic fungi after the introduction of Art. 59.1. In this paper, we summarize the recent advances and future potentials of taxonomic studies of coelomycetous studies

Keywords morphology – phylogeny – pleomorphism – species complexes
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Mycosphere publishes reviews, research articles, methodology papers, taxonomic works such as monographs, and checklists which are relevant to fungal biology, including lichens. The official journal language is English.

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